Time Kill Kinetic, Antibiofilm Activity and Effect on Biomolecules Release of Drypetes Gosswelleri and Echinops Giganteus Essential Oils on Bacteria

Main Article Content

Feudjieu Egoume Gaizirene
Tchinda Fossi Cedric
Kemegne Gislaine Aurelie
Moni Ndedi Esther Del Florence
Tchamgoue Deutou Armelle
Nana Wilfrid Gautier
Enyang Doreen
Agbor Agbor Gabriel

Abstract

Finding alternative strategies to confront bacterial resistance is an urgent need. Biofilm-forming bacteria have become a serious problem in medicine. Bacteria can use biofilm as a mechanism of resistance against antibacterial drugs. The aim of this study was to study times kill kinetic of Drypetes gosswelleri and Echinops giganteus essential oils, the antibiofilm formation activity and their effect on cell release compounds. Times kill kinetic was study by quantification of cellular growth over time in Petri dish after her inoculation by the cells treated with antibacteria at MBC and incubation at different times during 24h. The antibiofilm activity carried out by microdilution using 96 wells microplate. The production of biofilms by cells treated was observed and quantified after coloration by Crystal Violet dye and spectrophotometric dosage at 630nm. The effect on cell release compounds are study by determination of absorbent material in extracellular medium at 260nm after exposure at the antibacterial during 24h. The results obtained showed that, these EOs have an ability to kill bacterial cells over time, Drypetes gossweileri EOs like Ciprofloxacin, caused the death of all the cells in the inoculum treated before 14h of exposure of Staphylococcus aureus strain. Against Salmonella enteritidis strain, Drypetes gossweileri and Echnops giganteus EOs kill all the cells after 24h like the two antibiotics. One effect of the action these EOs on the strains elucidated in this study was the leakage of intracellular absorbent materials (DNA and RNA) this materialized a diminution of membrane permeability and cell wall integrity.

Article Details

How to Cite
Feudjieu Egoume Gaizirene, Tchinda Fossi Cedric, Kemegne Gislaine Aurelie, Moni Ndedi Esther Del Florence, Tchamgoue Deutou Armelle, Nana Wilfrid Gautier, Enyang Doreen, & Agbor Agbor Gabriel. (2023). Time Kill Kinetic, Antibiofilm Activity and Effect on Biomolecules Release of Drypetes Gosswelleri and Echinops Giganteus Essential Oils on Bacteria. International Journal of Medical Science and Clinical Research Studies, 3(07), 1265–1277. https://doi.org/10.47191/ijmscrs/v3-i7-08
Section
Articles

References

I. Basli A, Chibane M. 2012. Activité antibactérienne des polyphénols extraits d’une plante médicinale de la flore d’Algérie : Origanum glandulosum. Phytothérapie 10, 2–9.

II. Mosquera WG, Criado LY, Guerra BE. 2020. Antimicrobial activity of fungal endophytes of medicinal plants Mammea americana (Calophyllaceae), Moringa oleifera (Moringaceae). Biomédica, 40(1), 55 71.

III. Costerton JW, Geesey GG, Cheng KJ. 1978. How bacteria stick. Scientific American, 238(1), 86-95.

IV. Basem B, Anas R, Lama S, Jagjit SD, Khang WG, Long CM, Yaman K, Abdelhakim B, Mahibub MK, Chadi S. 2022. Evaluation of antibiofilm activity of Thymus syriacus essential oil against clinically isolated MDR bacteria. Progress in Microbes and Molecular Biolog, 5, 1 a0000284.

V. Lewis K. 2001. Riddle of biofilm resistance. Antimicrobial Agents and Chemotherapy, 45: 999-1007.

VI. Lewis K. 2005. Persister cells and the riddle of biofilm survival. Biochemistry (Mosc) 70, 267-274.

VII. Schuster M, Greenberg EP. 2006. A network of networks: quorum-sensing gene regulation in Pseudomonas aeruginosa. International Journal of Medical Microbiology, 296(2-3), 73-81.

VIII. Miloš N, Sava V, Jelena Đ, Olgica S, Ljiljana Č. 2014. Antibacterial and anti-biofilm activity of ginger (zingiber officinale (roscoe)) ethanolic extract. Kragujevac Journal of Science 36, 129-136.

IX. Gilbert P, McBain AJ, Rickard AH. 2003. Formation of microbial biofilm in hygienic situations: a problem of control. International Biodeterioration and Biodegradation 51, 245-248.

X. Anderson GG, O’Toole GA. 2008. Innate and induced resistance mechanisms of bacterial biofilms. Current Topics Microbiology and Immunology 322, 85–105.

XI. Barchan A, Bakkali M, Arakrak A, Laglaoui A. 2016. Antibacterial and anti-biofilm Effects of three species of Mentha: Mentha spicata, Mentha pulegium and Mentha piperita. Phytotherapie 14, 88-96.

XII. Costerton JW, Stewart PS, Greenberg EP. 1999. Bacterial biofilms: a common cause of persistent infections. Science 284, 1318-1322.

XIII. Driche EH, Nasserdine S, Christian B, Abdelghani Z, Frederic P, Florence M, Boubekeur B. 2017. Streptomyces sp. AT37 isolated from a Saharan soil produces a furanone derivative active against multidrug-resistant Staphylococcus aureus. World Journal of Microbiology and Biotechnology 33, 1-13

XIV. Caglar E, Tzora A, Skoufos I, Fotou K, Maloupa E, Grigoriadou K, Voidarou C, Zeugolis DI. 2023. The Assessment of Antimicrobial and Anti-Biofilm Activity of Essential Oils against Staphylococcus aureus Strains. Antibiotics, 12, 384.

XV. Gonzalez-Escobedo G, Marshall JM, Gunn JS. 2011. Chronic and acute infection of the gall bladder by Salmonella typhi: understanding the carrier state. National Review of Microbiology 9, 9-14.

XVI. Ma DS, Tan LT-H, Chan K-G, Wei HY, Priyia P, Lay-Hong C, Long CM, Trahir MK, Learn-Han L, Bey-Hing G. 2018. Resveratrol-potential antibacterial agent against foodborne pathogens. Frontiers in Pharmacology 9, 102.

XVII. Caillet S, Lacroix M. (2007). Les huiles essentielles : leurs propriétés antimicrobiennes et leurs applications potentielles en alimentaire, INRS-Institut Armand-Frappier, (RESALA), Canada , 1- 8.

XVIII. Quave CL, Plano LRW, Pantuso T, Bennett BC. 2008. Effects of extracts from Italian medicinal plants on planktonic growth, biofilm formation and adherence of methicillin-resistant Staphylococcus aureus. Journal of Ethnopharmacology 118, 418-428.

XIX. Sandasi M, Leonard CM, Viljoen AM. 2010. The in vitro antibiofilm activity of selected culinary herbs and medicinal plants against Listeria monocytogenes. Letters in Applied Microbiology 50(1), 30-35.

XX. Agnaniet H, Agrebi A, Bikanga R, Makani T, Lebibi J, Casabianca H, Morère A, Menut C. 2011. Essential oil of Plectranthus tenuicaulis leaves from Gabon, source of (R), (E)-6,7-Epoxyocimène. An unusual chemical composition within the Genus Plectranthus. National Productivity Council, 6: 409-416.

XXI. CLSI. 1998. Methods for determining bactericidal activity of antimicrobial agents. Approved Guideline, CSLI document M26-A. Clinical and laboratory standard institute, 950 West Valley Road Suite 2500, Wayne, Pennsylvania 19087.

XXII. Feudjieu EG, Kemegne GA, Tchinda FC, Tchamgoue DA, Moni Ndedi EDF, Matchuenkam GS, Agbor G. 2023. Synergistic Effects of Essential Oils and Antibiotics Against Some Bacterial Strains, Journal of Drug Delivery and Therapeutics 13,73-82.

XXIII. O’Toole GA. 2011. Microtiter dish biofilm formation assay. Journal of Visulized Experiments 30, 2437.

XXIV. Stiefel P, Rosenberg U, Schneider J, Stefan M, Maniura-Webber K, Ren Q. 2016. Is biofilm removal property assesed, Comparison of different quantification methods in a 96-weels plae system. Applied microbiology and biotechnology 100, 4135-4145.

XXV. Rhayour K, Bouchiki T, Tantaoui-Elaraki A, Sendide K, Remmal A. 2003. The mechanism of bactericidal action of oregano and clove essential oil and their phenolic major components on Escherichia coli and Bacillus cereus. Journal of Essential oil Research 15, 356-362.

XXVI. Bloomfield SF, Denyer SP, Hugo WB. 1991. Mechanisms of action of chemical biocides. Their study and exploitation. Methods for assessing antimicrobial activity.Technical series of the Society for Applied Bacteriology, Oxford, UK Blackwell Scientific Publications.

XXVII. Ratnakar P, Murthy S. 1996. Preliminary studies on the antitubercular activity and the mechanism of action of the water extract of garlic (Allium sativum) and its two partially purified proteins (garlic defensis). Indian Journal Clinical and Biochemistry 11, 37-41.

XXVIII. Moni NDEF, Assam AJP, Nyegue MA, Feudjieu EG, Penlap BV, Etoa F–X. 2018. Anti-mycobacterial efficacy of three essentials oils from medicinal plants currently used traditionally to treat tuberculosis in Cameroon. American Journal of Essential Oil and Natural Products 6, 10-18.

XXIX. Eyélé MC, Menut C, Bessière JM, Lamaty G, Nzé Ekekang L, Denamganai J. 1997. Aromatic Plants of Tropical Central Africa: Benzyl isothiocyanate as major constituent of bark essential oil of Drypetes gossweileri S. Moore. Journal of Essential Oil Research 9, 367-370.

XXX. Ndoye F, Tchinang TF, Nyegue AM, Abdou JP, Yaya AJ, Tchinda AT, Essame JO, Etoa F-X. 2016. Composition chimique, propriétés antioxydantes et anti-inflammatoires in vitro des huiles essentielles de quatre plantes diététiques et médicinales du Cameroun. BioMedical Central Complementary and Alternative Medecine 16, 117.

XXXI. Menut C, Lamaty G, Weyerstahl P, Marschall H, Seelmann I, Amvam

ZPH. 1997. Aromatic plants of tropical Central Africa. Part XXXI. Tricyclic sesquiterpenes from the root essential oil of Echinops giganteus var. lelyi C. D. Adams. Flavour Fragrance Journal 12, 415–421.

XXXII. Pavela R, Filippo M, Mbuntcha H, Woguem V, Dongmo FHP, Womeni HM, Azefack TL, Barboni L, Nicoletti M, Canale A, Benelli G. 2016. Traditional herbal remedies and dietary spices from Cameroon as novel sources of larvicides against filariasis mosquitoes. Parasitology Research, 4617–4626.

XXXIII. Xie C, Sun L, Meng L, Wang M, Xu J, Bartlam M, Guo Y. 2015. Sesquiterpenes from carpesium macrocephalum inhibits Candida albicans biofilm formation and dimorphism. Bioorganic and Medicinal Chemistry Letters 25, 09-11.

XXXIV. Wang L, Tamta T, Beatriz BA, Andrej T, Mercedes GM. 2020. Bateriophage-antibiotic combination against Ciprofloxacin and Ceftriaxone resistant Escherichia coli in vitro and in experimental Galleria mellonella model. International Journal of antimicrobiology agents 56.

XXXV. Muhammad Y, Debarum D, Mark DPW. 2021. Enchancement of antibiofilm activity of ciprofloxacin against Staphylococcus aureus by administration of antimicrobial paptidesm. Antibiotics 10, 1159.

XXXVI. Heurlier K, Denervaud V, Haas D. 2006. Impact of quorum sensing on fitness of Pseudomonas aeruginosa. International Journal of Medecal Microbiology 296, 93-102.

XXXVII. Høiby N, Bjarnsholt T, Givskov M, Molin S, Ciofu O. 2010b. Antibiotic resistance of bacterial biofilms. International Journal of Antimicrobial Agents 35, 322-332.

XXXVIII. Fuqua WC, Winans SC. 1994. A Luxr-Luxi type regulatory system activates agrobacterium ti plasmid conjugal transfer in the presence of a plant tumor metabolite. Journal of Bacteriology 176, 2796-2806.

XXXIX. Amaya S, José AP, Susana AB, Juan CV, Alicia BB, Mario EA. 2012. Inhibition of quorum sensing in Pseudomonas aeruginosa by sesquiterpene lactones. Phy-tomedicine, 1-5.

XL. Rice SA, Mc Dougald D, Kumar N, Kjelleberg S. 2005. The use of quorum-sensing blockers as therapeutic agents for the control of biofilm-associated infections. Current Opinion in Investigational Drugs 6, 178-184.

XLI. Famuyide IM, Aro AO, Fasina FO, Eloff JN, McGaw LJ. 2019. Antibacterial and antibiofilm activity of acetone leaf extracts of nine under-investigated south African Eugenia and Syzygium (Myrtaceae) species and their selectivity indices. Biomolecules complement alternative medecine 19, 1–13.

XLII. Borges A, Lúcia C, Simões AC, Maria J, Saavedra B, Manuel S. 2013. The action of selected isothiocyanates on bacterial biofilm prevention and control. International Biodeterioration and Biodegradation, 1-9.

XLIII. Elmasri WA, Mohamed-Elamir F, Hegazy B, Mina AA, Ekrem KA, Wail AC, Yehia MA, Abdul NHC, David BCA, Paul WPA. 2014. Biofilm blocking sesquiterpenes from Teucrium polium. Phytochemistry, 1-7

XLIV. Bajpai VK, Sharma A, andBaek KH. 2013. Antibacterial mode of action of Cudraniatricuspidata fruit essentialoil affecting membrane permeability and surface characteristics of food-bornepathogens. Food Control 32, 582–590.

XLV. Tchinang F, Ndoye FFM, Keumoe R, Zeuko’o ME, Fekam BF, Etoa FX. 2023. In vitro anti‑yeast activity, kinetics and mechanism of action of essential oils from two cameroonian medicinal plants. BMC Complementary Medicine and Therapies, 23:115.

XLVI. Moni NEDF, Nyegue MA, Assam Assam JP, Betote DPH, Feudjieu EG, Penlap BV, and Etoa F-X. 2019. Effects of Essential oil from Drypetes gossweileri S. Moore stem barks on Cell Release and DNA Synthesis of Mycobacterium tuberculosis. Journal of Drug Delivery and Therapeutics 9, 319-324.

XLVII. Bin Jiang, Fei Wang, Lei Liu, Shangyl Tian, Wenliang Li, Xiaoguang. 2017. Antibacterial activity and action mechanism of the Echinops ritro L. essential oil against foodborne pathogenic bacteria. Journal of essential oil bearing plant 20, 1172-1183.

XLVIII. Jayanta KP, Kwang-Hyun B. 2016. Antibacterial Activity and Action Mechanism of the Essential Oil from Enteromorpha linza L. against Foodborne Pathogenic Bacteria. Botanical Studies 56.

XLIX. Carson CF, Mee BJ, Riley TV. 2002. Mechanism of action of Melaleuca alternifolia (tea tree) oil on Staphylococcus aureus determined by time-kill, lysis, leakage, and salt tolerance assays and electron microscopy. Antimicrobial Agents and Chemotherapy 46, 1914–1920.

L. Ping li , Yi-meng, Cui wang, Hua-ping Zhu. 2021. Actibacterial activity and main action pathway of benzyl isothiocyanate extracted from papaya seeds. Journal of food Sciences 86, 169-176.

LI. Shuangshuang W, Siyu L, Guo H, Lili Z, Xin Lu, Haiyan W, Long W, Jiaying Zh, Wupeng G. 2022. Antimicrobial activity and mechanism action of isothiocyanate from Moringa oleifera seeds against Bacillus cereus and Cronobacter sakazakii and its application in goat milk. Food Control 139.

LII. Cowan MM. 1999. Plant products as antimicrobial agents. Clinical Microbiology Reviews 12, 564-582.

LIII. Sülsen VP, Puente V, Papademetrio D, Batlle A, Martino VS, Frank FM. 2016. Mode of Action of the Sesquiterpene Lactones Psilostachyin and Psilostachy in C on Trypanosoma cruzi. PLoS ONE 11.

LIV. Francois J, Chomarat M, Weber M, Gerard A. 2003. De l’antibiogramme à la prescription. Biomerieux 2éme édition, 8-22.

LV. Zhang y, Yewt WW. 2009. Mechanisms of drug resistance in Mycobacterium tuberculosis. International Journal of Tuberculosis and Lung Diseases 13, 1320–1330.

LVI. Abdallah R.B, Frikha D., Maalej S., Sassi S. 2019. In vitro evaluation of the antibacterial and antifungal activities of Marine algae, Journal of International Medecine 31, 38-44.

LVII. Kontiza I, Stavi M, Zloh M, Vagias C, Gibborns S, Roussis V. 2008. New metabolites with antibacterial activity from yhe marine angiosperm Cymodocea nosada. Tetrahedron 64, 1696-1702.